NanoBRET assays provide a sensitive way to detect protein interactions in living cells. Read more about BRET and learn how NanoLuc luciferase can be used to create sensitive BRET-based protein interactions assays. bit.ly/2rqF6wV | The Scientist
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NanoBRET assays provide a sensitive way to detect protein interactions in living cells. Read more about BRET and learn how NanoLuc luciferase can be used to create sensitive BRET-based protein interactions assays. bit.ly/2rqF6wV | The Scientist
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A luciferase assay is a bioluminescent technique used to measure gene expression, promoter strength, or protein activity. It works by using the luciferase enzyme to catalyze the oxidation of a substrate called luciferin, which produces light. The intensity of the light emitted is directly proportional to the amount of luciferase enzyme, which in turn reflects the activity of the gene or promoter being studied. | Tsouryel Ben Israel
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SOLVED: The enzyme called luciferase catalyzes the reaction that allows fireflies to emit light The first step in this reaction involves the substrate compound called luciferin , reacting with ATP. The luciferase enzyme has been crystallized bound to the product of this one-step reaction, as shown in the Pymol diagram below. (Dotted lines indicate stabilizing interactions between active site residues and the product:)HO_COzGln338luciferinNote:Lys529Blue nitrogen Red oxygen Orange sulfur or phos
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